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用于阴道内艾滋病毒暴露和艾滋病毒感染的人性化 NOG 小鼠
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Immunology and Infection
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JoVE Journal Immunology and Infection
Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection
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用于阴道内艾滋病毒暴露和艾滋病毒感染的人性化 NOG 小鼠

DOI:
10.3791/60723-v

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08:15 min

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January 31, 2020

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, , , , , , , , ,
1Department of Clinical Medicine,Aarhus University, 2Department of Infectious Diseases,Aarhus University Hospital, 3Department of Biomedicine,Aarhus University, 4Department of Gynecology and Obstetrics,Aarhus University Hospital, 5Department of Biology,University of Nebraska at Omaha

Chapters

  • 00:04Title
  • 00:50Cord Blood CCR5Δ32 Genetic Variant Screening
  • 01:50Intravaginal HIV Exposure
  • 03:20RNA Extraction, Complementary DNA (cDNA) Synthesis, and Viral RNA Quantification BY Droplet Digital Polymerase Chain Reaction (ddPCR)
  • 04:55Application of Combination Antiretroviral Therapy (cART)-Containing Chow
  • 05:42Results: Representative HIV infection and Treatment DATA
  • 07:13Conclusion

Summary

Automatic Translation

Automatic Translation

我们开发了一种基于干细胞移植、阴道内人体免疫缺陷病毒暴露和滴数字PCR RNA的人性化和人体免疫缺陷病毒感染NOG小鼠模型的生成和评估方案量化。

Tags

Humanized NOG MiceIntravaginal HIV ExposureHIV InfectionPreclinical HIV ResearchViral StrainsHIV InterventionsCCR5 VariantViral RNA QuantificationCord Blood ScreeningCCR5 Delta 32HIV Exposure Protocol

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