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Journal
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Immunology and Infection
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通过生命内显微镜在斑马鱼胚胎的分孔细菌感染过程中巨噬细胞细胞死亡的可视化
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Visualization of Macrophage Lytic Cell Death During Mycobacterial Infection in Zebrafish Embryos via Intravital Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
通过生命内显微镜在斑马鱼胚胎的分孔细菌感染过程中巨噬细胞细胞死亡的可视化
DOI:
10.3791/60698-v
•
06:49 min
•
January 09, 2019
•
Liangfei Niu
,
Cong Wang
,
Kaile Zhang
2
,
Miaomiao Kang
2
,
Rui Liang
,
Xiaonan Zhang
,
Bo Yan
1
Shanghai Public Health Clinical Center
,
Fudan University
,
2
School of Life Sciences
,
Bengbu Medical College
Chapters
00:04
Title
00:55
M. marinum
Single Cell Inoculum Preparation
02:01
Infection via Bacterial Microinjection
04:16
Live Imaging of the Infection
05:13
Results: Morphological Changes in
M. marinum
Infection Induced Macrophage Lytic Cell Death and UV Induced Macrophage Apoptosis
06:19
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一种技术,用于可视化在胚胎斑马鱼的巨噬行为和死亡在
分枝杆菌
感染期间。包括制备细菌、胚胎感染和生命内显微镜的步骤。在涉及感染或无菌炎症的类似情况下,此技术可应用于观察细胞行为和死亡。
Tags
Macrophage
Lytic Cell Death
Mycobacterial Infection
Zebrafish Embryos
Intravital Microscopy
Cell Death Visualization
Live Imaging
Mycobacterium Marinum
Agarose Mounting
Intramuscular Infection
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