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Journal
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Immunology and Infection
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原虫性原虫感染に対する原発星およびミクログリアの同時分離
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection
Please note that all translations are automatically generated.
Click here for the English version.
原虫性原虫感染に対する原発星およびミクログリアの同時分離
DOI:
10.3791/60680-v
•
09:34 min
•
March 18, 2020
•
Aline de Oliveira Lima Pacheco*
1
,
Marcelo Pires Amaral*
1
,
Ingrid Sancho de Farias
,
Luiza Zainotti Miguel Fahur Bottino
,
Karina Ramalho Bortoluci
1
Centro de Terapia Celular e Molecular (CTCMol)
,
Universidade Federal de São Paulo (UNIFESP)
,
2
Departamento de Ciências Biológicas, Centro de Terapia Celular e Molecular (CTCMol)
,
Universidade Federal de São Paulo (UNIFESP)
Chapters
00:04
Introduction
01:31
Glial Cells Extraction, Maintenance, and Dissociation
07:28
Results: Astrocytes and Microglia Infection
08:32
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルの全体的な目標は、マウスアストロサイトおよびミクログリア細胞を中枢神経系から抽出、維持、解離し、続いて原虫寄生虫に感染する方法を指示することである。
Tags
Astrocyte Isolation
Microglia Isolation
Glial Cell Extraction
Central Nervous System
Immunological Context
Alzheimer’s
Parkinson’s
Protozoa Parasite
Virus Infection
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