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超结构保存细胞内膜蛋白定位电子显微镜分析的CryoAPEX方法
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The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells
Please note that all translations are automatically generated.
Click here for the English version.
超结构保存细胞内膜蛋白定位电子显微镜分析的CryoAPEX方法
DOI:
10.3791/60677-v
•
11:45 min
•
February 27, 2020
•
Elaine M. Mihelc
,
Stephanie Angel
,
Robert V. Stahelin
3,4
,
Seema Mattoo
4,5,6
1
Department of Biological Sciences
,
Purdue University
,
2
Department of Medicinal Chemistry and Molecular Pharmacology
,
Purdue University
,
3
Purdue Institute of Inflammation, Immunology and Infectious Disease
,
Purdue University
,
4
Bindley Biosciences Center
,
Purdue University
,
5
Purdue Institute of Integrative Neuroscience
,
Purdue University
,
6
Purdue Center for Cancer Research
,
Purdue University
Chapters
00:05
Title
01:36
Transfection, Chemical Fixation and Peroxidase Reaction
03:48
High Pressure Freezing
04:46
Freeze Substitution
06:23
Resin Infiltration and Embedding
08:11
Sectioning and TEM Imaging
09:17
Results: TEM Images
11:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了CryoAPEX方法,其中APEX2标记膜蛋白可以通过传输电子显微镜在最佳保存的细胞超结构内进行局部化。
Tags
CryoAPEX
Electron Microscopy
Membrane Protein Localization
Ultrastructural Preservation
APEX2
Cryofixation
Freeze Substitution
Virus Replication
Bacterial Toxin
Golgi Fragmentation
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