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Immunology and Infection
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从高致病性H5N1病毒和禽流感病毒中生产含有包络糖蛋白的高蒂特感染性流感伪型颗粒
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses
Please note that all translations are automatically generated.
Click here for the English version.
从高致病性H5N1病毒和禽流感病毒中生产含有包络糖蛋白的高蒂特感染性流感伪型颗粒
DOI:
10.3791/60663-v
•
08:10 min
•
January 15, 2020
•
Fengwei Zhang
,
Yanan Wang
,
Xuechai Shang
,
Shanshan Wang
,
Rong Xiao
,
Hongjuan Zhou
,
Long Cai
1
Central Laboratory
,
Hang Zhou Red Cross Hospital
,
2
Clinical Laboratory
,
Yuhang District Maternity and Child Health Care Hospital
Chapters
00:04
Title
00:39
Four-plasmid Cotransfection Mediated by Lipofection
02:15
Susceptible Cell Seeding and Pseudotyped Viral Particle Quantification
04:01
Infectivity Assay
05:34
Results: Infectivity Assay of Pseudotyped Particles
06:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一个实验过程,以产生高蒂特传染性病毒伪型颗粒(pp),包络糖蛋白从两个流感A毒株,以及如何确定其传染性。该协议具有很强的适应性,可开发具有不同包络糖蛋白的任何其他类型的包络病毒的pps。
Tags
Influenza
Pseudotyped Particles
Viral Envelope Glycoproteins
H5N1
H7N9
BSL-2
Transfection
QRT-PCR
Virus Reassortment
Cell Culture
Incubation
Harvest
Quantification
Benzonase Nuclease
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