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在 Vivo 感染利什曼病亚马孙病评估小鼠的寄生虫毒性
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
In Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice
DOI:

06:57 min

February 20, 2020

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Chapters

  • 00:04Introduction
  • 01:16In Vitro Differentiation of L. amazonensis Promastigotes into Axenic Amastigotes
  • 01:56C57BL/6 Footpad Infection with L. amazonensis
  • 02:47Mouse Footpad Lesion Extraction and Parasite Load Determination
  • 04:29Results: LIR1 Knockout In Vivo Lesion Development and Intracellular Replication
  • 06:03Conclusion

Summary

Automatic Translation

在这里,我们提出了一个编译的协议,以评估小鼠与利什曼病的皮感染。这是研究寄生虫毒性的可靠方法,允许对脊椎动物宿主对感染的反应进行系统观察。

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