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ショウジョウバエ
幼虫における大鼻細胞の解剖と脂質液滴染色
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JoVE Journal
Biology
Dissection and Lipid Droplet Staining of Oenocytes in
Drosophila
Larvae
Please note that all translations are automatically generated.
Click here for the English version.
ショウジョウバエ
幼虫における大鼻細胞の解剖と脂質液滴染色
DOI:
10.3791/60606-v
•
05:37 min
•
December 28, 2019
•
Chuanxian Wei*
1
,
Yan Yan*
1
,
Xiaoli Miao
,
Renjie Jiao
2,3
1
Sino-French Hoffmann Institute, School of Basic Medical Sciences
,
Guangzhou Medical University
,
2
The Second Affiliated Hospital of Guangzhou Medical University
,
3
The State Key Laboratory of Respiratory Disease
,
Guangzhou Medical University
Chapters
00:04
Title
00:32
Egg Laying
01:13
Starvation Treatment
02:10
Oenocyte Dissection
03:10
Lipid Droplet Staining
04:02
Mounting and Imaging
04:41
Results: Representative Lipid Droplet Staining
05:06
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
ここで提示する、脂質液滴特異的蛍光色素であるBODIPY 493/503を用いた
ショウジョウバエ
幼虫における卵細胞の解剖および脂質液滴染色に関する詳細な方法である。
Tags
Oenocyte
Lipid Droplet
Drosophila
Larvae
Dissection
Staining
Starvation
Metabolism
Epidermis
PBS
Fixation Buffer
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