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In Vivo Aumento da Indução de CélulaS T Reguladoras de Gut-Homing
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
DOI:

08:02 min

January 22, 2020

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Chapters

  • 00:05Title
  • 00:571-alpha-Hydroxylase and Retinaldehyde Dehydrogenase 2 (RALDH2) Lentivirus (Lenti-CYP-ALDH virus) Preparation
  • 03:04Bone Marrow-Derived Dendritic Cell (BMDC) Generation
  • 05:06BMDC Lenti-CYP-ALDH Transduction
  • 06:00Results: Representative Gut-Homing Regulatory T Cell Induction
  • 07:38Conclusion

Summary

Automatic Translation

Aqui apresentamos um protocolo para o aumento in vivo da ininvitro-homing indução de células T regulamentares. Neste protocolo, as células dendríticas são projetadas para produzir localmente altas concentrações da vitamina D ativa (1,25-dihidroxivitamina D ou 1,25[OH]2D) e a vitamina A ativa (ácido retinoico ou AR) de novo.

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