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Immunology and Infection
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压力处理下细菌生长的多尺度分析
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Multi-scale Analysis of Bacterial Growth Under Stress Treatments
Please note that all translations are automatically generated.
Click here for the English version.
压力处理下细菌生长的多尺度分析
DOI:
10.3791/60576-v
•
12:08 min
•
November 28, 2019
•
Julien Cayron
,
Christian Lesterlin
1
Molecular Microbiology and Structural Biochemistry (MMSB)
,
University of Lyon 1, CNRS, Inserm
Chapters
00:04
Title
01:03
Cell Culture, Stress-induction, and Sampling Procedure
02:40
Plating Assay and Flow Cytometry
04:55
Snapshot Microscopy Imaging
05:49
Microfluidics Time-lapse Microscopy Imaging
08:19
Image Analysis
09:38
Results: Flow Cytometry and Microscopy Analysis
11:31
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议允许在单细胞和细胞群水平上对在应激条件下的细菌生长进行时间解析的描述。
Tags
Bacterial Growth
Stress Treatment
Multi-scale Analysis
Cell Viability
Morphology
DNA Content
Population Recovery
Pharmaceutical Industry
Antimicrobial Screening
Dose-dependent
Time-lapse Microscopy
Dilution And Plating Assay
Flow Cytometry
Microscopy Snapshot
Cephalexin
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