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冠状血管生成体模型
JoVE Journal
Medicine
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JoVE Journal
Medicine
In Vitro Model of Coronary Angiogenesis
Please note that all translations are automatically generated.
Click here for the English version.
冠状血管生成体模型
DOI:
10.3791/60558-v
•
08:03 min
•
March 10, 2020
•
Colton L. Large
,
Halie E. Vitali
,
Jeffery D. Whatley
,
Kristy Red-Horse
,
Bikram Sharma
1
Department of Biology
,
Ball State University
,
2
Department of Biology
,
Stanford University
Chapters
00:04
Introduction
00:38
Embryo Harvesting
01:28
E11.5 Embryo Heart Isolation
02:14
Sinus Venosus (SV) and Ventricle Isolation
03:09
SV and Whole Ventricle Culture
04:09
Vascular Endothelial Growth Factor-A (VEGF-A) Treatment
04:54
Fixation, Immunostaining, Mounting, and Analysis
06:22
Results: Representative Coronary Angiogenesis Evaluation
07:12
Conclusion
Summary
Automatic Translation
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Automatic Translation
冠状血管生成体外模型可用于发现冠状血管生成细胞和分子机制。正月线和内卡组织体外外培养物在响应VEGF-A时表现出强劲的生长,并表现出与体内类似的COUP-TFII表达模式。
Tags
In Vitro
Coronary Angiogenesis
Embryonic Day 11.5
Pregnant Mouse
Uterine Horn
Dorsal Aorta
Vein
Sinus Venosus
Ventricles
Cell Culture
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