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Postnatal Fare Serebellum prominin-1+ Kök Hücrelerin Safifikasyon
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Purification of Prominin-1+ Stem Cells from Postnatal Mouse Cerebellum
DOI:

07:04 min

April 12, 2020

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Chapters

  • 00:05Introduction
  • 00:53Cerebellum Dissection and Cell Suspension Preparation
  • 02:34Immunolabeling of Stem Cells
  • 03:44Magnetic Column Preparation and Cell Sorting
  • 04:22Passaging of Neurospheres and Differentiation
  • 05:27Results: Prominin-1 Stem Cells Isolated from Postnatal Cerebellum
  • 06:15Conclusion

Summary

Automatic Translation

Burada gösterilen, postnatal fare beyincik beyaz madde kök hücreleri arındırmak, kültür ve ayırt etmek için etkili ve uygun maliyetli bir yöntemdir.

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