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Immunology and Infection
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使用抗生素耐药性平台进行抗生素脱除
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Antibiotic Dereplication Using the Antibiotic Resistance Platform
Please note that all translations are automatically generated.
Click here for the English version.
使用抗生素耐药性平台进行抗生素脱除
DOI:
10.3791/60536-v
•
10:49 min
•
October 17, 2019
•
Haley L. Zubyk
,
Georgina Cox
,
Gerard D. Wright
1
Department of Biochemistry and Biomedical Sciences, M.G. DeGroote Institute for Infectious Disease Research
,
McMaster University
,
2
Department of Molecular and Cellular Biology
,
University of Guelph
Chapters
00:04
Title
00:38
ARP/MARP Frozen Stock Library Plate Preparation
01:53
Seed Culture and Replication Plate Preparation
04:29
Dereplication Plate MHB Overlay and ARP/MARP Library Plate Preparation
06:51
Dereplicating using the ARP/MARP
07:59
Results: Dereplication Results
09:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个平台,它利用异源性抗生素耐药
大肠杆菌
库来消除抗生素。细菌或真菌产生的抗生素的特性可以通过表达其各自抗药性基因的
大肠杆菌
的生长来推断出来。该平台经济高效且具有时间效率。
Tags
Antibiotic Dereplication
Antibiotic Resistance Platform
Template Customization
Beta-lactam
E. Coli
Cation Adjusted MHB
ARP
MARP
96 Deep Well Plate
Glycerol
Streptomyces
Antibiotic Medium
Bennett’s Agar
Sterility Control
Article
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