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光遺伝学的変調型心筋細胞活性の電気機械評価
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity
Please note that all translations are automatically generated.
Click here for the English version.
光遺伝学的変調型心筋細胞活性の電気機械評価
DOI:
10.3791/60490-v
•
12:52 min
•
March 05, 2020
•
Ramona A. Kopton
2,3
,
Cinthia Buchmann
2
,
Robin Moss
2
,
Peter Kohl
2
,
Rémi Peyronnet
2
,
Franziska Schneider-Warme
2
1
Institute for Experimental Cardiovascular Medicine, University Heart Center Freiburg-Bad Krozingen
,
Medical Center-University of Freiburg
,
2
Faculty of Medicine
,
University of Freiburg
,
3
Faculty of Biology
,
University of Freiburg
Chapters
00:05
Introduction
01:33
Cell Isolation
02:57
Cardiomyocyte Culture
04:17
Patch-Clamp Experiment
05:44
Carbon Fiber Preparation
07:40
Cardiomyocyte Contraction Force Recording
10:23
Results: Representative Patch-Clamp and Carbon Fiber Recordings
11:44
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
ウサギ心筋細胞におけるGtACR1活性化の電気機械効果を評価するプロトコルを提示する。細胞分離、培養、アデノウイルス伝達、パッチクランプ、炭素繊維技術による機能実験に関する詳細な情報を提供します。
Tags
Optogenetics
Cardiomyocyte
Electromechanical Assessment
GtACR1
Patch Clamp
Sarcomere Tracking
Carbon Fiber
Optical Defibrillation
Ventricular Cardiomyocytes
Smooth Muscle
Single-cell Muscle
Cell Stretching
Cell Dissociation
Cell Culture
Laminin
Article
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