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小鼠膀胱肿瘤器官的培养、操作和正交移植
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids
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小鼠膀胱肿瘤器官的培养、操作和正交移植

DOI:
10.3791/60469-v

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08:43 min

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January 31, 2020

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, , ,
1Department of Life Sciences,Pohang University of Science and Technology

Chapters

  • 00:04Title
  • 00:50In Vitro Culture of Bladder Tumor Organoids
  • 03:50Genetic Manipulation of Bladder Organoids via Lentivirus-mediated Transduction
  • 04:36Orthotopic Transplantation of Bladder Organoids
  • 07:06Results: Culture and Transplantation of Bladder Tumor Organoids
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

该协议提供了详细的实验步骤，以建立从致癌性鼠膀胱癌衍生的膀胱肿瘤器官的三维体外培养。介绍了肿瘤器官的传体内、遗传工程、正交移植等培养方法。

Tags

Tumor OrganoidsBladder CancerCell CultureGenetic ManipulationOrthotopic TransplantationTumor Microenvironment

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