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Medicine
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跟踪超副磁性氧化铁标记的美感血干细胞使用MRI后,创伤性脑损伤Murine模型内伤分娩
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Tracking Superparamagnetic Iron Oxide-labeled Mesenchymal Stem Cells using MRI after Intranasal Delivery in a Traumatic Brain Injury Murine Model
Please note that all translations are automatically generated.
Click here for the English version.
跟踪超副磁性氧化铁标记的美感血干细胞使用MRI后,创伤性脑损伤Murine模型内伤分娩
DOI:
10.3791/60450-v
•
10:03 min
•
November 21, 2019
•
Rami Ahmad Shahror
2,3
,
Chung-Che Wu
3,4,5
,
Yung-Hsiao Chiang
2,3,4,5
,
Kai-Yun Chen
2,3
1
Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology
,
Taipei Medical University and National Health Research Institutes
,
2
Center for Neurotrauma and Neuroregeneration
,
Taipei Medical University
,
3
TMU Neuroscience Research Center
,
Taipei Medical University
,
4
Department of Neurosurgery
,
Taipei Medical University Hospital
,
5
Department of Surgery, School of Medicine, College of Medicine
,
Taipei Medical University
Chapters
00:05
Title
01:06
Superparamagnetic Iron Oxide (SPIO) nanoParticles labeling of Mesenchymal Stem Cells (MSC)
01:44
Controlled Cortical Impact (CCI) Injury
04:04
Intranasal MSC Delivery
05:57
In Vivo Magnetic Resonance Imaging (MRI)
06:54
MSC Tracking and Quantification
08:03
Results: Representative SPIO-Labeled MSC Detection and Tracking
09:08
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里介绍的是一个非侵入性的中伤性干细胞(MSC)传递和跟踪的小鼠模型创伤性脑损伤的协议。超对磁氧化铁纳米颗粒用作磁共振成像(MRI)探头,用于MSC标签和非侵入性体内跟踪,使用实时MRI进行鼻内传递。
Tags
Superparamagnetic Iron Oxide
Mesenchymal Stem Cells
MRI
Intranasal Delivery
Traumatic Brain Injury
Murine Model
Non-invasive Tracking
Therapeutic Effects
Cell Labeling
Controlled Cortical Impact Injury
Stereotactic Frame
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