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1Department of Marine Biology and Ecology, Rosenstiel School of Marine and Atmospheric Science,University of Miami, 2Department of Biology,University of Miami, 3The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, and Regenerative Medicine and Stem Cell Research Center,Ben Gurion University of the Negev
Chapters
- 00:04Introduction
- 00:36Fluorescence-Activated Cell Sorting (FACS) Gating Strategy Setup and Analysis
- 01:49FACS Sorting and Cell Collection
- 02:55Results: Representative Live Coral Cell Gating
- 03:59Conclusion
Os corais criam ecossistemas biodiversos importantes para humanos e organismos marinhos. No entanto, ainda não entendemos todo o potencial e função de muitas células de coral. Aqui, apresentamos um protocolo desenvolvido para o isolamento, rotulagem e separação de populações de células de corais pedregosos.
