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原发性慢性淋巴细胞白血病细胞的细胞次细胞分段监测核/细胞质蛋白贩运
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking
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原发性慢性淋巴细胞白血病细胞的细胞次细胞分段监测核/细胞质蛋白贩运

DOI:
10.3791/60426-v

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11:39 min

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October 23, 2019

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, , ,
1Paul O’Gorman Leukaemia Research Centre, Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences,University of Glasgow

Chapters

  • 00:05Title
  • 01:22Isolation of Chronic Lymphocytic Leukemia (CLL) Cells from Patient Blood Samples
  • 03:23Preparation of Subcellular Fractions from CLL Cells
  • 05:54Preparation of Whole Cell Lysates (WCL) from CLL Cells
  • 06:32Downstream Analysis of Subcellular Fractions
  • 08:59Results: Analysis of Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells
  • 10:47Conclusion

Summary

Automatic Translation

Automatic Translation

该协议能够优化和随后有效地从原发性慢性淋巴细胞白血病细胞中生成核质和细胞质部分。这些样本用于确定蛋白质定位以及细胞刺激和药物治疗后核质室和细胞质室之间的蛋白质贩运变化。

Tags

Subcellular FractionationChronic Lymphocytic LeukemiaNuclear/cytoplasmic Protein TraffickingTumor Suppressor ProteinsPathogenesisB Cell MalignanciesProtein LocalizationProtein MovementDensity Gradient CentrifugationMononuclear Cells

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