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Journal
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Immunology and Infection
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第四组病毒SSHHPS使用体外和西里科方法的分析
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
Please note that all translations are automatically generated.
Click here for the English version.
第四组病毒SSHHPS使用体外和西里科方法的分析
DOI:
10.3791/60421-v
•
10:40 min
•
December 21, 2019
•
Xin Hu
,
Jaimee R. Compton
,
Patricia M. Legler
1
National Center for Advancing Translational Sciences
,
National Institutes of Health
,
2
United States Naval Research Laboratory
Chapters
00:05
Title
01:01
Identification of SSHHPS in the Host Genome Using BLAST
01:49
Design and Preparation of Protease Substrates
04:07
Continuous and Discontinuous Enzyme Assays
06:02
Docking Substrate Peptides to the VEEV-nsP2 Cysteine Protease
08:27
Results: SSHHPS Analysis
09:54
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们提出了一个通用方案,用于识别嵌入在病毒多蛋白中的同源宿主病原体蛋白序列(SSHHPS)的短段。SSHHPS被病毒蛋白酶识别,并指导几种IV组病毒对特定宿主蛋白的定向破坏。
Tags
Group IV Viral Genomes
Viral Protease Cleavage Sites
Host Protein Sequences
Immune Response
Protease Assays
Zika SSHHPS Sequences
Brain And Eye Development
Protein BLAST
PHI-BLAST
CFP-YFP Substrates
Bacterial Expression
Protein Purification
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