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Immunology and Infection
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铁限分枝杆菌产生的细胞外囊泡的分离和特征
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Immunology and Infection
Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria
Please note that all translations are automatically generated.
Click here for the English version.
铁限分枝杆菌产生的细胞外囊泡的分离和特征
DOI:
10.3791/60359-v
•
08:27 min
•
October 31, 2019
•
Shamba Gupta
,
G. Marcela Rodriguez
1
Public Health Research Institute, New Jersey Medical School
,
Rutgers, The State University of New Jersey
Chapters
00:04
Title
00:47
Preparation of Iron-depleted Defined Medium
01:58
Growing Mycobacteria in Iron-limited Conditions
03:44
Collection, Isolation and Quantification of MEVs (Mycobacterial Extracellular Vesicles)
06:38
Results: MEV Purification and Quantification
07:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
结核分枝杆菌
显示,由于低铁条件,细胞外囊泡的产量增加并释放。这项工作详细介绍了产生低铁条件的协议,以及针对缺铁而释放的分菌外囊泡的纯化和表征方法。
Tags
Extracellular Vesicles
Mycobacteria
Iron-limited
Membrane Vesicles
Mycobacterium Tuberculosis
Mycobacterium Smegmatis
Minimal Medium
Metal Chelating Resin
Bacterial Culture
Agar Plates
Spectrophotometry
Late Logarithmic Phase
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