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前列腺有机体培养作为工具，将基因型和突变谱转化为药理反应
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JoVE Journal Cancer Research
Prostate Organoid Cultures as Tools to Translate Genotypes and Mutational Profiles to Pharmacological Responses
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前列腺有机体培养作为工具，将基因型和突变谱转化为药理反应

DOI:
10.3791/60346-v

•

08:36 min

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October 24, 2019

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, , ,
1Human Oncology and Pathogenesis Program,Memorial Sloan Kettering Cancer Center, 2Howard Hughes Medical Institute

Chapters

  • 00:05Title
  • 01:17Isolation, Enzymatic Digestion, and Establishments of Organoids
  • 03:06Assessing Organoid Formation Capacity
  • 04:05Determining Pharmacological Responses of Organoids
  • 05:44Results: Organoid Seeding Efficiency and Pharmacological Response
  • 07:41Conclusion

Summary

Automatic Translation

Automatic Translation

这里介绍的是一个协议，研究在前列腺上皮器官的药理反应。有机体与体内生物学非常相似，并重述了患者基因，使它们成为有吸引力的模型系统。前列腺器官可以从野生型前列腺、基因工程小鼠模型、良性人体组织和晚期前列腺癌中建立。

Tags

Prostate Organoid CulturesPharmacological ResponsesGenotypeMutational ProfilesIn Vitro SystemThree-dimensional StructureBasement Membrane MatrixDrug ResistanceDome Plating MethodSingle Cell SuspensionCell CountingDownstream ApplicationsTrypsin ReplacementCell Dissociation

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