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球状外生长作为前维沃分析分析涉及的路径的表皮上皮细胞激活
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Biology
Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation
Please note that all translations are automatically generated.
Click here for the English version.
球状外生长作为前维沃分析分析涉及的路径的表皮上皮细胞激活
DOI:
10.3791/60324-v
•
06:39 min
•
August 19, 2020
•
Jennifer Eymael
,
Laura Miesen
,
Fieke Mooren
,
Jitske Jansen
2
,
Jack Wetzels
,
Johan van der Vlag
,
Bart Smeets
1
Radboud Institute for Molecular Life Sciences, Department of Pathology
,
Radboud University Medical Center
,
2
Radboud Institute for Molecular Life Sciences, Amalia Children’s Hospital, Department of Paediatric Nephrology
,
Radboud University Medical Center
,
3
Radboud Institute for Health Sciences, Department of Nephrology
,
Radboud University Medical Center
,
4
Radboud Institute for Molecular Life Sciences, Department of Nephrology
,
Radboud University Medical Center
Chapters
00:04
Introduction
00:39
Mouse Kidney Dissection
01:04
Isolation of Glomeruli from Mouse Kidney
02:25
Culturing of Glomerular Outgrowth
03:40
Analysis of Parietal Epithelial Cell Proliferation
04:47
Results: Analysis of Glomerular Outgrowth
06:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了一种培养和分析从小鼠肾脏分离出的封装球状上皮细胞生长的方法。此方法可用于研究涉及单骨上皮细胞增殖和迁移的途径。
Tags
Glomerular Outgrowth
Parietal Epithelial Cell Activation
Kidney
Ex Vivo Assay
Cellular Processes
Primary Cells
Glomeruli
Kidney Isolation
Cell Culture
Microscopy
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