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Journal
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Immunology and Infection
/
成像人类免疫学突触
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Imaging the Human Immunological Synapse
Please note that all translations are automatically generated.
Click here for the English version.
成像人类免疫学突触
DOI:
10.3791/60312-v
•
09:37 min
•
December 26, 2019
•
Chapters
00:05
Title
01:26
Adhesion of Raji Cells to the Chamber Slides and 7-amino-4-chloromethylcoumarin (CMAC, Cell Tracker Blue) Labeling
03:31
Pulse of CMAC labeled Raji Cells with Staphylococcal Enterotoxin E and Preparation of Jurkat Cells
04:51
Co-seeding of Raji and Jurkat Cells and Time Lapse Imaging of Emerging Synaptic Conjugates
05:51
End Point Formation of Synaptic Conjugates and Fixation
06:38
Results: Imaging the Human Immunological Synapse
08:28
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议图像免疫突触形成和随后的极化分泌流量对免疫突触。细胞结合形成于超抗原脉冲拉吉细胞(作为抗原呈现细胞)和Jurkat克隆(作为效应帮助剂T淋巴细胞)之间。
Tags
Immunological Synapse
Cell-to-cell Conjugation
Antigen-presenting Cell
T-helper Lymphocyte
Time-lapse Acquisition
Wide-field Fluorescence Microscopy
Image Processing
Cell Fixation
Immunofluorescence Staining
Laser Scanning Fluorescent Microscopy
Fibronectin
Raji Cells
Cell Tracker Blue
Cell Suspension
Article
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