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Journal
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Cancer Research
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小肠神经内分泌肿瘤类球体的建立与特征
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Cancer Research
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JoVE Journal
Cancer Research
Establishment and Characterization of Small Bowel Neuroendocrine Tumor Spheroids
Please note that all translations are automatically generated.
Click here for the English version.
小肠神经内分泌肿瘤类球体的建立与特征
DOI:
10.3791/60303-v
•
09:43 min
•
October 14, 2019
•
Po Hien Ear
,
Guiying Li
,
Meng Wu
,
Ellen Abusada
,
Andrew M. Bellizzi
,
James R. Howe
1
Department of Surgery
,
University of Iowa Carver College of Medicine
,
2
High Throughput Screening Facility
,
University of Iowa Carver College of Medicine
,
3
Department of Pathology
,
University of Iowa Carver College of Medicine
Chapters
00:04
Title
00:44
Small Bowel Neuroendocrine Tumor (SBNET) Collection and Cell Dissociation
02:10
Culture of SBNET as Tumor Spheroids in ECM
04:02
Characterization of SBNET Spheroids by Immunofluorescence
05:50
Splitting SBNET Spheroids
07:23
Results: Immunofluorescence Imaging and Drug Testing of SBNET Spheroids
09:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
神经内分泌肿瘤(NETs)来自神经峰的神经内分泌细胞。它们生长缓慢,对文化充满挑战。我们提出了一个替代策略,通过培养它们作为球体,从小肠中种植NET。这些球体有小肠NET标记,可用于药物测试。
Tags
Small Bowel Neuroendocrine Tumor
SBNET
3D Culture
Spheroids
Cell Line
Neuroendocrine Tumor
Cell Isolation
Cell Digestion
Cell Counting
Extracellular Matrix
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