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Journal
/
Immunology and Infection
/
在
伪单体
中生成帧内基因删除突变体,并在简单小鼠感染模型中测试病毒衰减
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Generation of In-Frame Gene Deletion Mutants in
Pseudomonas aeruginosa
and Testing for Virulence Attenuation in a Simple Mouse Model of Infection
Please note that all translations are automatically generated.
Click here for the English version.
在
伪单体
中生成帧内基因删除突变体,并在简单小鼠感染模型中测试病毒衰减
DOI:
10.3791/60297-v
•
08:34 min
•
January 08, 2020
•
Meagan E. Valentine
,
Brandon D. Kirby
,
Hongwei D. Yu
2
1
Progenesis Technologies, LLC
,
2
Department of Biomedical Sciences, Pediatrics
,
Joan C. Edwards School of Medicine at Marshall University
Chapters
00:05
Title
01:24
Detection of Double-crossover Recombinants of
P. aeruginosa
02:24
Confirmation of Gene Deletion with Colony PCR
03:07
Visualization of Bioluminescence-labeled Bacteria in Mice
05:42
Inoculation of Animals with Bacterial Strains
06:28
Results: Evaluation of Attenuated
P. aeruginosa
Strain
07:24
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了一个简单和可重复的小鼠感染模型协议,以评估与美国食品和药物管理局(FDA)批准的
大肠杆菌
用于商业用途的假
单
抗病毒的遗传改性菌株的衰减。
Tags
Pseudomonas Aeruginosa
In-frame Gene Deletion
Virulence Attenuation
Mouse Infection Model
Reproducibility
Crossover Recombinant
Colony PCR
Double Crossover Recombinant
Carbenicillin Sensitivity
Sucrose Resistance
Gene Mutation
Article
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