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通过注射药物加载的脂体将药物定位到拉瓦尔斑马鱼巨噬细胞
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Immunology and Infection
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JoVE Journal Immunology and Infection
Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes
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通过注射药物加载的脂体将药物定位到拉瓦尔斑马鱼巨噬细胞

DOI:
10.3791/60198-v

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11:31 min

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February 18, 2020

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, , , , ,
1Department of Molecular Medicine and Pathology, Faculty of Medical and Health Sciences,University of Auckland, 2School of Pharmacy, Faculty of Medical and Health Sciences,University of Auckland, 3School of Medicine, Faculty of Medical and Health Sciences,University of Auckland

Chapters

  • 00:05Introduction
  • 01:20Drug-loaded Marina Blue-Labeled Lipsome Preparation
  • 03:24Liposome Size and Zeta Potential Characterization and Entrapment Efficiency and Drug Loading Calculation
  • 04:11Preparation and Liposome Injection
  • 06:47Confocal Imaging and Image Analysis
  • 09:03Results: Representative Effects of Drug-loaded Lipsome Injection
  • 10:48Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了药物加载脂体及其显微注射到幼虫斑马鱼的合成，目的是将药物输送到巨噬细胞。

Tags

LiposomesMacrophagesZebrafishDrug DeliveryMitochondrial Reactive Oxygen SpeciesPoloxamer 188ExtrusionUltracentrifugationLive Cell Imaging

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