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1Department of Biomedical and Molecular Sciences and Department of Pathology and Molecular Medicine,Queen’s University, Kingston, 2Department of Chemical and Physical Sciences,University of Toronto, Mississauga, 3Department of Laboratory Medicine, Brampton Civic Hospital Campus,William Osler Health System, 4Latner Thoracic Surgery Research Laboratories
Chapters
- 00:05Introduction
- 01:24Plating and Differentiation Induction of HC11 Cells
- 03:363D Growth and Differentiation Induction of EpH4 Cells in EHS Matrix
- 06:24Western Blotting for β-Casein
- 07:42Results: Assessment of HC11 and EpH4 Cell Differentiation
- 08:49Conclusion
Descriviamo tecniche per l'induzione della differenziazione di due linee epiteliali mammarie, HC11 ed EpH4. Mentre entrambi richiedono siero di vitello fetale, insulina e prolattina per produrre proteine del latte, le cellule EpH4 possono differenziarsi completamente in mammosfere in coltura tridimensionale. Questi modelli complementari sono utili per gli studi di trasduzione del segnale di differenziazione e neostrulasia.
