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在臭氧暴露后阿尔韦拉巨噬细胞的Vivo评估中
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Immunology and Infection
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JoVE Journal Immunology and Infection
In Vivo Assessment of Alveolar Macrophage Efferocytosis Following Ozone Exposure
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在臭氧暴露后阿尔韦拉巨噬细胞的Vivo评估中

DOI:
10.3791/60109-v

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08:54 min

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October 22, 2019

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, , ,
1Department of Pharmacology and Toxicology,East Carolina University, 2Research Triangle Park,National Institute of Environmental Health and Sciences

Chapters

  • 00:04Title
  • 01:17Preparation of Jurkat T Cell Line
  • 03:41Murine Oropharyngeal Instillation of Apoptotic Cells
  • 04:45Bronchoalveolar Lavage Fluid Collection and Processing
  • 06:30Calculation of Alveolar Macrophage Efferocytic Index
  • 07:06Results: O3 Exposure Induces Pulmonary Inflammation and Injury
  • 08:14Conclusion

Summary

Automatic Translation

Automatic Translation

本手稿描述了一个协议，用于确定暴露于臭氧（一种标准空气污染物）是否会损害体内的藻体细胞体细胞化。该协议利用常用试剂和技术，可适用于多种肺损伤模型，以确定对肺泡巨噬细胞细胞病的影响。

Tags

Alveolar MacrophageEfferocytosisOzone ExposureLung InjuryInflammationJurkat T CellsApoptotic CellsUV IrradiationCell CultureIn Vivo Assessment

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