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外迁移系统中淋巴细胞迁移的评估
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Immunology and Infection
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JoVE Journal Immunology and Infection
Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
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外迁移系统中淋巴细胞迁移的评估

DOI:
10.3791/60060-v

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10:25 min

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September 20, 2019

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,
1Department of Internal Medicine,University of Utah, 2Research Service,VA Nebraska Iowa Health Care System, 3Department of Internal Medicine,University of Nebraska Medical Center, 4Department of Environmental, Agricultural & Occupational Health,University of Nebraska Medical Center

Chapters

  • 00:04Title
  • 00:50Isolation of CD4+ T Cells from Spleens and Lungs of OVA-challenged Mice
  • 04:25Ex vivo Transmigration Procedure
  • 06:39Quantification of Ex vivo Transmigration
  • 08:06Results: Assessment of Lymphocyte Migration under Various Conditions
  • 09:42Conclusion

Summary

Automatic Translation

Automatic Translation

在此协议中，淋巴细胞被放置在迁移系统的顶室中，由多孔膜与底部腔室隔开。化学因子被添加到底部腔室，从而诱导沿化学基质梯度的活性迁移。48小时后，淋巴细胞在两个腔室计数，以定量迁移。

Tags

Lymphocyte MigrationTransmigrationImmune ResponseChemokinesILC2Ex VivoCell IsolationCell CountingCell Suspension

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