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Journal
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Immunology and Infection
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从人类和小鼠心肌标本中分离巨噬细胞子集和细胞位细胞
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Immunology and Infection
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Immunology and Infection
Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
Please note that all translations are automatically generated.
Click here for the English version.
从人类和小鼠心肌标本中分离巨噬细胞子集和细胞位细胞
DOI:
10.3791/60015-v
•
07:25 min
•
December 17, 2019
•
Geetika Bajpai
,
Kory J. Lavine
2,3
1
Department of Medicine
,
Washington University School of Medicine
,
2
Department of Immunology and Pathology
,
Washington University School of Medicine
,
3
Department of Developmental Biology
,
Washington University School of Medicine
Chapters
00:05
Title
01:14
Preparation of Human Cardiac Tissue Specimens
01:41
Preparation of the Mouse Heart
02:11
Preparation of Single Cell Suspension
04:50
Antibody Staining
05:33
Results: Flow Cytometry Gating Scheme
06:22
Conclusion
Summary
Automatic Translation
English (Original)
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这里介绍的一个协议,通过酶消化制备单个细胞悬浮液,从人类和小鼠心肌中分离出巨噬细胞和其他非免疫细胞的各种子集。提出了基于流细胞学的识别与分离巨噬细胞表征的门控方案。
Tags
Macrophage Subsets
Stromal Cells
Cardiac Inflammation
Cardiac Repair
Immune Cell Diversity
Single Cell Suspension
Viable Immune Cells
Nonimmune Cell Populations
Murine Heart
Collagenase I
DNase I
Hyaluronidase
DMEM
Digestion
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