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Journal
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Immunology and Infection
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在自身免疫糖尿病模型中高效生成抗原特异性原小鼠细胞毒性T细胞细胞细胞,用于功能测试
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Immunology and Infection
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JoVE Journal
Immunology and Infection
High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model
Please note that all translations are automatically generated.
Click here for the English version.
在自身免疫糖尿病模型中高效生成抗原特异性原小鼠细胞毒性T细胞细胞细胞,用于功能测试
DOI:
10.3791/59985-v
•
11:31 min
•
August 16, 2019
•
Howard W. Davidson
,
Joseph Ray Cepeda
,
Nitin S. Sekhar
,
Junying Han
,
Ling Gao
,
Tomasz Sosinowski
,
Li Zhang
1
Barbara Davis Center for Childhood Diabetes
,
University of Colorado Denver
,
2
Department of Medicine, Endocrinology, Diabetes & Metabolism
,
Baylor College of Medicine
,
3
Scientific Center
,
Shandong Provincial Hospital affiliated to Shandong University
Chapters
00:04
Title
01:00
Transfection of Viral Producer Cells
03:34
Primary CD8 T Cell Isolation and Activation
07:10
Transduction of Activated CD8 T Cells
09:29
Results: Analysis of Transduced CD8 CAR-T Cells
10:42
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文介绍了一种用于生成抗原特异性CD8 T细胞及其在体外扩张的协议,目的是产生大量功能性T细胞,用于体外和体内。
Tags
Antigen-specific
Primary Mouse
Cytotoxic T Cells
Autoimmune Diabetes
High-efficiency
Transduction
CAR T Cells
Cancer
Immune Cells
Phoenix Cells
Transfection
Reduced Serum Medium
Packaging Plasmid
Virus
IL-2
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