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Journal
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Immunology and Infection
/
原人类单核细胞的隔离、转染和文化
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Isolation, Transfection, and Culture of Primary Human Monocytes
Please note that all translations are automatically generated.
Click here for the English version.
原人类单核细胞的隔离、转染和文化
DOI:
10.3791/59967-v
•
09:13 min
•
December 16, 2019
•
Karlie Plaisance-Bonstaff
2
,
Celeste Faia
,
Dorota Wyczechowska
,
Duane Jeansonne
,
Cecilia Vittori
3
,
Francesca Peruzzi
2
1
Department of Medicine
,
Louisiana State University Health Sciences Center
,
2
Department of Microbiology, Immunology, and Parasitology
,
Louisiana State University Health Sciences Center
,
3
Department of Biomedical and Clinical Sciences L. Sacco
,
University of Milan
Chapters
00:05
Title
00:53
Primary Human Monocyte Isolation
03:22
Primary Human Monocyte Culture
04:25
Primary Human Monocyte Transfection
05:53
M1/M2 Differentiation and Activation
07:07
Results: Representative Transfection Efficiency, Viability, and Protein Regulation Effectivity
08:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里介绍的是一个优化的协议,用于分离、培养、转染和区分人类原发性单核细胞与艾滋病毒感染者和健康对照。
Tags
Primary Human Monocytes
Isolation
Transfection
Culture
HIV Infection
Immune Dysfunction
Antiretroviral Therapy
Molecular Mechanisms
Biosafety
EDTA
Magnetic Beads
PBS
Centrifugation
Cell Counting
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