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Journal
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Immunology and Infection
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人类干细胞衍生的伊拉尔肠细胞单层中M细胞样细胞的诱导分化
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers
Please note that all translations are automatically generated.
Click here for the English version.
人类干细胞衍生的伊拉尔肠细胞单层中M细胞样细胞的诱导分化
DOI:
10.3791/59894-v
•
11:34 min
•
July 26, 2019
•
Alyssa C. Fasciano
,
Sarah E. Blutt
,
Mary K. Estes
,
Joan Mecsas
1
Program in Immunology
,
Sackler School of Graduate Biomedical Sciences
,
2
Department of Molecular Virology and Microbiology
,
Baylor College of Medicine
,
3
Department of Molecular Biology and Microbiology
,
Tufts University
Chapters
00:04
Title
00:27
Inducing M Cell Differentiation in Human Ileal Enteroid-derived Monolayers
05:46
Verifying M Cell Differentiation
09:30
Results: Immunofluorescence of M Cells in Human Ileal Enteroid-derived Monolayers
11:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了如何诱导M细胞在人类干细胞衍生的卵单层中的分化,以及评估其发育的方法。
Tags
M Cells
Intestinal Stem Cells
Induced Differentiation
Ileal Enteroid Monolayers
Extracellular Matrix
Transwell Membranes
EDTA
Trypsin
Cell Disaggregation
Single Cells
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