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使用预突子形成测定使用预突子组织者珠和"神经元球"文化
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture
Please note that all translations are automatically generated.
Click here for the English version.
使用预突子形成测定使用预突子组织者珠和"神经元球"文化
DOI:
10.3791/59893-v
•
10:17 min
•
August 02, 2019
•
Shumaia Parvin
,
Renoma Takeda
,
Yukio Sasaki
1
Functional Structure Biology Laboratory, Department of Medical Life Science
,
Yokohama City University Graduate School of Medical Life Science
Chapters
00:04
Title
00:55
Preparation of Neuron Balls as Hanging Drop Culture
03:24
Placing Neuron Balls on PLL-Coated Glass Coverslips and Culture Maintenance
04:29
Applying LRRTM2 Beads on Neuron Ball Culture With or Without Cell Bodies
06:07
Fluorescence Microscopy
07:19
Results: Analysis of Accumulation of Presynaptic Proteins in LRRTM2-Induced Presynapses of Axons of Neuron Ball Culture
09:15
Conclusion
Summary
Automatic Translation
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Automatic Translation
预突生形成是一个动态过程,包括以适当的顺序积累突触蛋白。在这种方法中,前突子形成由与"神经元球"培养的正交片上与前突子组织者蛋白结合的珠子触发,因此在先发形成期间很容易分析突触蛋白的积累。
Tags
Presynapse Formation
Presynapse Organizer Beads
Neuron Ball Culture
Synapse Formation
Presynaptic Proteins
Cortical Neuron Culture
Embryonic Mouse Brain
Trypsinization
Trituration
Hanging Drop Culture
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