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Journal
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Immunology and Infection
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毛里小肠内皮淋巴细胞的内质成像
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine
Please note that all translations are automatically generated.
Click here for the English version.
毛里小肠内皮淋巴细胞的内质成像
DOI:
10.3791/59853-v
•
08:00 min
•
June 24, 2019
•
Luo Jia
,
Karen L. Edelblum
1
Center for Immunity and Inflammation, Department of Pathology, Immunology and Laboratory Medicine
,
Rutgers New Jersey Medical School
Chapters
00:04
Title
00:58
Intestinal Mucosa Exposure
03:41
Spinning Disk Confocal Microscopy Imaging
05:59
Results: Representative Gamma-delta Intraepithelial Lymphocyte (IEL) Behavior Analyses
07:15
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一种通过倒转旋转盘共聚焦显微镜,利用鼠小肠的细胞内成像来可视化GFP标记的IELs的方法。该技术能够跟踪粘液中的活细胞长达4小时,并可用于研究各种肠道免疫-皮皮相互作用。
Tags
Intravital Imaging
Intraepithelial Lymphocytes
Murine Small Intestine
Microscopy
Immune Cell Interactions
Epithelium
Real-time Imaging
Mucosal Immunity
Epithelial Biology
Cancer Biology
Surgical Technique
Anesthetized Transgenic Mouse
EGFP
Gamma-delta T-cell Receptor
Hoechst 33342 Dye
Abdominal Incision
Mesentery
Peritoneal Cavity
Suture Placement
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