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Journal
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Immunology and Infection
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使用胶原酶E从毛里结肠分离拉米纳普里亚单核细胞
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E
Please note that all translations are automatically generated.
Click here for the English version.
使用胶原酶E从毛里结肠分离拉米纳普里亚单核细胞
DOI:
10.3791/59821-v
•
09:48 min
•
September 26, 2019
•
Duneia McManus
2,3,4
,
Horacio J. Novaira
2,3,4
,
Anouk A.J. Hamers
2,3,4
,
Asha B. Pillai
2,3,4,5
1
Department of Pediatrics, Division of Hematology / Oncology and Bone Marrow Transplantation
,
University of Miami Miller School of Medicine
,
2
Batchelor Children’s Research Institute
,
University of Miami Miller School of Medicine
,
3
Department of Microbiology & Immunology
,
University of Miami Miller School of Medicine
,
4
Sylvester Comprehensive Cancer Center
,
University of Miami Miller School of Medicine
,
5
Holtz Children’s Hospital
,
University of Miami Miller School of Medicine
Chapters
00:04
Title
01:00
Harvesting the Colon
01:32
Cleaning the Colon
03:10
Collagenase Digestion 1
03:34
Silica-Based Density Separation Media Gradient Separation
05:44
Collect Mononuclear Cells from the Gradient Interface
06:39
Results: Isolation of Lamina Propria Mononuclear Cells
08:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议的目标是通过使用胶原酶对组织进行酶消化,分离位于结肠层状的单核细胞。该协议允许对单核细胞进行有效分离,从而产生单个细胞悬浮液,进而可用于强健的免疫分体。
Tags
Colon
Lamina Propria
Mononuclear Cells
Collagenase
Immune Cells
Intestine
Inflammation
Mouse Models
Cancer
Allergy
Transplantation
Autoimmunity
Phenotypic Analysis
Flow Cytometry
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