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Immunology and Infection
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李斯特菌单细胞基因
感染后小鼠脾脏原位干扰素伽马生产的成像
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following
Listeria monocytogenes
Infection
Please note that all translations are automatically generated.
Click here for the English version.
李斯特菌单细胞基因
感染后小鼠脾脏原位干扰素伽马生产的成像
DOI:
10.3791/59819-v
•
09:11 min
•
July 16, 2019
•
Julie M. Mazet
,
Ana L. Chiodetti
,
Jagdish N. Mahale
,
Audrey Gérard
1
The Kennedy Institute of Rheumatology
,
The University of Oxford
Chapters
00:04
Title
01:03
Listeria monocytogenes
(LM) Infection and Brefeldin A (BFA) Treatment
02:02
Spleen Harvesting, Fixation with Paraformaldehyde (PFA), Freezing and Sectioning
04:41
Immunofluorescent Staining and Imaging
06:44
Results: IFNγ Production
08:19
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述了一个简单的共聚焦成像方法,以可视化细胞在原位定位,在鼠次淋巴器官中分泌细胞因子干扰素伽马的细胞。该协议可以扩展用于不同组织中其他细胞因子的可视化。
Tags
In Situ
Interferon Gamma
Listeria Monocytogenes
Mouse Spleen
Cytokine Visualization
Microenvironment
LM OVA Culture
Intravenous Injection
BFA
Euthanization
Spleen Dissection
Fixative Solution
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