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Journal
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Immunology and Infection
/
链球菌
中高分子质量蛋白的纯化
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Purification of a High Molecular Mass Protein in
Streptococcus mutans
Please note that all translations are automatically generated.
Click here for the English version.
链球菌
中高分子质量蛋白的纯化
DOI:
10.3791/59804-v
•
09:51 min
•
September 14, 2019
•
Takatoshi Murata
,
Mamiko Yamashita
,
Masao Ishikawa
2
,
Koji Shibuya
,
Nobuhiro Hanada
1
Department of Translational Research
,
Tsurumi University School of Dental Medicine
,
2
Laboratory for Oral Health Science
Chapters
00:06
Title
00:51
PCR Amplification
02:38
Cell Transformation
03:35
Purification of Polyhistidine-tagged GTF-SI
07:29
Results: Generation of
S. mutans
His-gtfC and Functional Restoration
08:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里描述的是一个简单的方法,用于纯化
链球菌
中的基因产物。该技术在蛋白质,特别是膜蛋白和高分子质量蛋白的纯化方面可能有利,可用于其他各种细菌物种。
Tags
Protein Purification
Streptococcus Mutans
High Molecular Mass Protein
PCR
Genomic DNA Extraction
Gel Electrophoresis
Gel Extraction
Nested PCR
Electroporation
Competent Cells
Brain-heart Infusion Broth
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