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使用荧光宏镜检查,通过"完整小鼠头骨"进行脑脊液传输的 Vivo 成像
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Neuroscience
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JoVE Journal Neuroscience
In Vivo Imaging of Cerebrospinal Fluid Transport through the Intact Mouse Skull using Fluorescence Macroscopy
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使用荧光宏镜检查,通过"完整小鼠头骨"进行脑脊液传输的 Vivo 成像

DOI:
10.3791/59774-v

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06:22 min

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July 29, 2019

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, , , , , , , , , ,
1Center for Translational Neuromedicine,University of Rochester Medical Center, 2Center for Translational Neuromedicine,University of Copenhagen

Chapters

  • 00:04Title
  • 00:55Surgical Preparation
  • 03:11Imaging Preparation
  • 04:10Fluorescent Cerebrospinal Fluid (CSF) Tracer Infusion and Imaging
  • 04:37Results: Representative Transcranial Microscopic Imaging
  • 05:40Conclusion

Summary

Automatic Translation

Automatic Translation

颅内光学成像允许通过完整的头骨在活小鼠皮层中对脑脊液进行宽场成像。

Tags

In VivoCerebrospinal Fluid TransportFluorescence MacroscopyGlymphatic TransportCisterna Magna CannulaHead PlateStereotactic FrameAnalgesiaDental CementCyanoacrylate Glue

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