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用于量化Vibrio Fischeri分离物之间竞争交互的联合孵化测定
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Immunology and Infection
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JoVE Journal Immunology and Infection
Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates
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用于量化Vibrio Fischeri分离物之间竞争交互的联合孵化测定

DOI:
10.3791/59759-v

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07:43 min

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July 22, 2019

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,
1Department of Marine Sciences,University of North Carolina, Chapel Hill

Chapters

  • 00:04Title
  • 00:28Preparation of Each Bacterial Strain for Incubation
  • 01:21Coincubation of Strains
  • 02:54Serial Dilution for Starting Inoculum
  • 04:37Taking T Final Measurements
  • 05:36Results: Quantifying Competitive Interactions Between the Strains
  • 07:26Conclusion

Summary

Automatic Translation

Automatic Translation

细菌编码各种机制,参与细菌间竞争。在这里,我们提出了一个基于培养的协议,用于描述细菌分离物之间的竞争相互作用,以及它们如何影响混合种群的空间结构。

Tags

Vibrio FischeriCoincubation AssayCompetitive InteractionsAntibiotic ResistanceFluorescent ProteinsCell SuspensionOptical DensityNormalizationDifferential TaggingColony Forming UnitsFluorescence Microscopy

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