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Journal
/
Neuroscience
/
从单体和体内使用的Α-syr核 in 预成型纤维的产生
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Generation of Alpha-Synuclein Preformed Fibrils from Monomers and Use In Vivo
Please note that all translations are automatically generated.
Click here for the English version.
从单体和体内使用的Α-syr核 in 预成型纤维的产生
DOI:
10.3791/59758-v
•
09:44 min
•
June 02, 2019
•
Joseph R. Patterson
,
Nicole K. Polinski
,
Megan F. Duffy
3
,
Christopher J. Kemp
,
Kelvin C. Luk
,
Laura A. Volpicelli-Daley
,
Nicholas M. Kanaan
,
Caryl E. Sortwell
3,6
1
Department of Translational Science and Molecular Medicine
,
Michigan State University
,
2
The Michael J. Fox Foundation for Parkinson’s Research
,
3
Neuroscience Program
,
Michigan State University
,
4
Center for Neurodegenerative Disease Research, Department of Pathology and Laboratory Medicine
,
University of Pennsylvania Perelman School of Medicine
,
5
Center for Neurodegeneration and Experimental Therapeutics
,
University of Alabama at Birmingham
,
6
Mercy Health Hauenstein Neuroscience Medical Center
Chapters
00:04
Title
01:13
Formation of α-synuclein Preformed Fibrils from Monomers
02:20
Sonication of α-synuclein Preformed Fibrils
04:37
Preparation of Custom Glass Needle Syringes for Stereotactic Injections
07:18
Results: Analysis of the Generated Alpha-Synuclein Preformed Fibrils
09:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文的目的是概述从单聚α-合核蛋白中产生纤维所需的步骤、随后的质量控制以及在体内使用预成型纤维。
Tags
Alpha-synuclein
Preformed Fibrils
Parkinson’s Disease
Synucleinopathy
Monomers
In Vivo
Cell Culture
Sonication
Centrifugation
Protocol
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