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Immunology and Infection
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用于狂犬病诊断的泛源病毒实时RT-PCR
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
Please note that all translations are automatically generated.
Click here for the English version.
用于狂犬病诊断的泛源病毒实时RT-PCR
DOI:
10.3791/59709-v
•
06:25 min
•
July 10, 2019
•
Denise A. Marston
,
Daisy L. Jennings
,
Nikki C. MacLaren
,
Daniel Dorey-Robinson
,
Anthony R. Fooks
2
,
Ashley C. Banyard
,
Lorraine M. McElhinney
2
1
Wildlife Zoonoses & Vector-Borne Diseases Research Group
,
Animal and Plant Health Agency
,
2
Institute of Infection and Global Health
,
University of Liverpool
Chapters
00:04
Title
00:58
Preparation of RNA Dilution Series
01:22
Real-time PCR
03:33
Data Analysis
04:28
Results: Pan-lyssavirus RT-PCR
05:38
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这种使用dsDNA间染的实时RT-PCR适用于诊断裂解病毒感染。该方法从从狂犬病疑似前身或死后样本中提取的RNA开始,详细说明主混合物制备、RNA添加、实时机器的设置以及结果的正确解释。
Tags
Pan-Lyssavirus
Real Time RT-PCR
Rabies Diagnosis
Lyssavirus Genus
Molecular Assays
RNA Quantification
PCR Master Mix
Beta-Actin
SYBR Green
Dissociation Curve
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