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Journal
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Immunology and Infection
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将鼠皮皮和皮米与热解辛分离,以检测部位特异性炎症mRNA和蛋白质
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein
Please note that all translations are automatically generated.
Click here for the English version.
将鼠皮皮和皮米与热解辛分离,以检测部位特异性炎症mRNA和蛋白质
DOI:
10.3791/59708-v
•
08:45 min
•
September 29, 2021
•
Vikramsingh Gujar*
1
,
Michael B. Anderson*
1
,
Kenneth E. Miller*
1
,
Radhika D. Pande
,
Pranav Nawani
,
Subhas Das
1
Department of Anatomy and Cell Biology
,
Oklahoma State University Center for Health Sciences
,
2
Institute for Shock Physics
,
Washington State University
Chapters
00:04
Introduction
01:21
Carrageenan-Induced Inflammation (C-II)
02:31
Thermolysin Epidermis and Dermis Separation
04:11
Tinctorial Histology
05:43
Results: Representative Site-Specific Inflammatory mRNA and Protein Detection
07:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里介绍的是一个表皮与真皮分离的协议,用于评估炎症调解器的产生。炎症后,大鼠后爪表皮在4°C下由热利辛从真皮中分离出来。 然后,通过RT-PCR进行表皮分析,通过西方污点和免疫造血术进行蛋白质评估。
Tags
Epidermis
Dermis
Thermolysin
Skin Inflammation
Inflammatory Mediators
Neurotrophins
Wound Healing
Skin Disease
Chronic Wounds
Burns
Epithelial Surfaces
GI Tract
Cornea
Sprague Dawley Rat
Lambda Carrageenan
Edema
Glabrous Hind Paw
Microdissection Forceps
DMEM
Stratum Corneum
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