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单核细胞化学策略活性在Vivo的定量和血液单核细胞衍生巨噬细胞的特征
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages
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单核细胞化学策略活性在Vivo的定量和血液单核细胞衍生巨噬细胞的特征

DOI:
10.3791/59706-v

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09:57 min

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August 12, 2019

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, ,
1Department of Internal Medicine, Section on Molecular Medicine,Wake Forest School of Medicine, 2Department of Biochemistry,Wake Forest School of Medicine

Chapters

  • 00:04Title
  • 00:47Preparation of the Monocyte Chemoattractant Protein (MCP)-1-loaded Growth Factor-reduced Basement Membrane-derived Solution
  • 01:40Basement Membrane-derived Solution Injection and Gel Plug Harvest
  • 03:29Basement Membrane-derived Gel Plug Digestion
  • 04:43Single-cell Western Blot (scWB) Analysis
  • 06:38scWB Chip Stripping
  • 07:35Results: Representative Basement Membrane-derived Gel Plug Cell Recruitment Analyses
  • 09:22Conclusion

Summary

Automatic Translation

Automatic Translation

在这里,我们提出一个协议,以量化小鼠模型中血液单核细胞的化学活性,评估营养、药理和遗传干预对血液单核细胞的影响,并描述在小鼠模型中产生的血单核细胞使用单核细胞-化学吸引蛋白-1(MCP-1)加载的基底膜衍生凝胶塞的小鼠模型。

Tags

Monocyte Chemotactic ActivityIn Vivo QuantificationMonocyte-derived MacrophagesSingle Cell AnalysisOmics TechniquesBasement Membrane-derived SolutionMCP-1Subcutaneous InjectionFibrous CapsuleGel Plug

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