Renal Subcapsular Transplantation of 2'-Deoxyguanosine-Treated Murine Embryonic Thymus in Nude Mice

Jianxue Wang; Guolin Chen; Qianwen Cui; Erfei Song; Weihua Tao; Wanqun Chen; Cunchuan Wang; Shiqi Jia

doi:10.3791/59657

Journal

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Immunology and Infection

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Renale Subcapsulaire transplantatie van 2 '-Deoxyguanosine-behandelde Muriene embryonale Thymus in naakt muizen

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Renal Subcapsular Transplantation of 2′-Deoxyguanosine-Treated Murine Embryonic Thymus in Nude Mice

Please note that all translations are automatically generated. Click here for the English version.

Renale Subcapsulaire transplantatie van 2 '-Deoxyguanosine-behandelde Muriene embryonale Thymus in naakt muizen

DOI:

10.3791/59657-v

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07:39 min

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July 19, 2019

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Jianxue Wang^3,4, Guolin Chen^3,4, Qianwen Cui^3,4, Erfei Song^3,4, Weihua Tao, Wanqun Chen, Cunchuan Wang^3,4, Shiqi Jia^3,4

¹The First Affiliated Hospital,Jinan University, ²Department of Biochemistry and Molecular Biology, School of Basic Medical Science,Jinan University, ³The Institute of Clinical Medicine,Jinan University, ⁴Joint institute of Metabolic Medicine Between Jinan University and University of Hong Kong

Chapters

00:04Title
01:06Embryonic Thymus Isolation
02:54Isolated Embryonic Thymus Culture and Renal Subcapsular Space Establishment
04:36Embryonic Murine Thymus Transplant
05:59Results: Thymus-Transplant Nude Mouse Peripheral Blood T Cell Identification and Evaluation
06:59Conclusion

Summary

Automatic Translation

Wij bieden een eenvoudige en efficiënte methode voor het transplantatie van 2 '-deoxyguanosine behandeld E 18.5 Thymus in de niercapsule van een naakt muis. Deze methode moet worden assistent in de studie van zowel Thymus epitheliale cellen functie en T-cellen rijping.