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木尿造血干细胞和祖细胞和MLL-AF9驱动白血病线粒体反应氧物种的流细胞学分析

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JoVE Journal Cancer Research

Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia

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木尿造血干细胞和祖细胞和MLL-AF9驱动白血病线粒体反应氧物种的流细胞学分析

DOI:

10.3791/59593-v

•

09:44 min

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September 05, 2019

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Daniela Di Marcantonio, Stephen M. Sykes

¹Blood Cell Development and Function Program,Fox Chase Cancer Center

Chapters

00:05Title
01:46Mitochondrial ROS Fluorogenic Dye Staining
04:41Lineage Antibody Staining
06:04Flow Cytometry Acquisition and Analysis
07:24Results: Flow Cytometry Gating Strategies and Mitochondrial ROS Levels
08:54Conclusion

Summary

Automatic Translation

我们描述了一种使用多参数流细胞测定法来检测小鼠健康造血干细胞和祖细胞（HSPCs）中的线粒体活性氧物种（ROS）和白血病细胞的方法，这些细胞来自急性骨髓性白血病（AML）的小鼠模型。MLL-AF9。

Tags

Flow Cytometry Mitochondrial Reactive Oxygen Species Hematopoietic Stem Cells Hematopoietic Progenitor Cells Acute Myeloid Leukemia MLL-AF9 Redox State Metabolism

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