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Medicine
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从成年小鼠分离心膜肌细胞
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Isolation of Atrial Myocytes from Adult Mice
Please note that all translations are automatically generated.
Click here for the English version.
从成年小鼠分离心膜肌细胞
DOI:
10.3791/59588-v
•
08:34 min
•
July 25, 2019
•
Hailey J. Jansen
,
Robert A. Rose
1
Libin Cardiovascular Institute of Alberta, Department of Cardiac Sciences, Department of Physiology and Pharmacology, Cumming School of Medicine
,
University of Calgary
Chapters
00:04
Title
00:42
Dissection of Mouse Atrial Appendage(s)
02:46
Isolation of Atrial Myocytes
06:19
Results: Representative Patch-clamp Data Obtained From Isolated Mouse Atrial Myocytes
07:52
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议用于使用块消化方法从成年小鼠心脏分离单个心房心肌细胞。这种方法用于分离右心房或左心房肌细胞,可用于在贴片夹研究中描述心房肌细胞电生理学。
Tags
Atrial Myocytes
Patch-clamp
Atrial Electrophysiology
Arrhythmogenesis
Trunk Digestion
Mouse Euthanasia
Atrial Appendage
Modified Tyrode’s Solution
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