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液滴数字 TRAP (ddTRAP): 对端粒重复放大协议的适应性, 以实现液滴数字聚合酶链反应
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JoVE Journal
Cancer Research
Droplet Digital TRAP (ddTRAP): Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction
Please note that all translations are automatically generated.
Click here for the English version.
液滴数字 TRAP (ddTRAP): 对端粒重复放大协议的适应性, 以实现液滴数字聚合酶链反应
DOI:
10.3791/59550-v
•
06:38 min
•
May 03, 2019
•
Mohammed E. Sayed
,
Aaron L. Slusher
,
Andrew T. Ludlow
1
School of Kinesiology
,
University of Michigan
Chapters
00:04
Title
00:27
Cell Lysis and Telomerase Extension Reaction
01:43
Droplet Digital PCR Setup
03:21
Detection of Telomerase Extension Products and Data Analysis
05:03
Results: Telomerase Activity
05:51
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们成功地转换了标准端粒重复扩增协议 (TRAP) 检测, 用于液滴数字聚合酶链反应。这种新的检测方法称为 ddTRAP, 更灵敏、更定量, 可以更好地检测和统计各种人体细胞内端粒酶活性。
Tags
DdTRAP
Telomere Repeat Amplification Protocol
Droplet Digital PCR
Telomerase Activity
Cell Lysis
Extension Reaction
DdPCR Master Mix
Droplet Generation
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