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In Vivo Intracerebral Stereotaxic Injections for Optogenetic Stimulation of Long-Range Inputs in Mouse Brain Slices
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
In Vivo Intracerebral Stereotaxic Injections for Optogenetic Stimulation of Long-Range Inputs in Mouse Brain Slices
DOI:

09:07 min

September 20, 2019

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Chapters

  • 00:04Title
  • 00:42Stereotaxic Surgery
  • 04:01Preparation of Brain Slices
  • 05:28Whole-Cell Patch-Clamp Recording
  • 07:21Results: Presubicular Layer III Neuron: Intrinsic Properties, Responses to Light Stimulation, and Post-hoc Revelation of Cell Morphology
  • 08:19Conclusion

Summary

Automatic Translation

Este protocolo describe un conjunto de métodos para identificar la conectividad funcional específica de tipo celular de entradas de largo alcance de regiones cerebrales distantes utilizando estimulaciones optogenéticas en rebanadas cerebrales ex vivo.

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