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小鼠胰腺胰岛细胞亚群中的钙动力学成像
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Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells
Please note that all translations are automatically generated.
Click here for the English version.
小鼠胰腺胰岛细胞亚群中的钙动力学成像
DOI:
10.3791/59491-v
•
08:03 min
•
November 26, 2019
•
Alexander Hamilton
2
,
Elisa Vergari
,
Caroline Miranda
,
Andrei I. Tarasov
4,5
1
Oxford Centre for Diabetes, Endocrinology and Metabolism
,
University of Oxford
,
2
Lund University Diabetes Centre, Unit of Molecular Metabolism, Clinical Research Centre
,
Malmö University HospitalMalmö
,
3
Institute of Neuroscience of Physiology, Department of Physiology, Metabolic Research Unit
,
University of Göteborg
,
4
Oxford National Institute for Health Research
,
Biomedical Research Centre
,
5
Life and Medical Sciences
,
University of Hertfordshire
Chapters
00:04
Title
00:30
Calcium (Ca
2+
) Imaging Dynamics
02:21
Microscope Setup
03:48
Time-Lapse Imaging
04:45
Numerical Data Analysis
06:16
Results: Representative Islet Cell Responses to Various Stimuli
07:26
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一个协议,用于在异质细胞群(如胰腺胰岛细胞)中成像和量化钙动力学。荧光光被输送到小岛内细胞的外围层,然后固定和成像,并每细胞对荧光强度的动态进行分析。
Tags
Pancreatic Islet Cells
Calcium Dynamics
Imaging
Cell Immobilization
Microscopy
Perfusion
Fluorescence Imaging
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