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利用成像质谱技术捕获组织与细胞之间的小分子通信
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JoVE Journal
Cancer Research
Capturing Small Molecule Communication Between Tissues and Cells Using Imaging Mass Spectrometry
Please note that all translations are automatically generated.
Click here for the English version.
利用成像质谱技术捕获组织与细胞之间的小分子通信
DOI:
10.3791/59490-v
•
07:58 min
•
April 03, 2019
•
Katherine E. Zink
,
Matthew Dean
2
,
Joanna E. Burdette
,
Laura M. Sanchez
1
Department of Medicinal Chemistry and Pharmacognosy
,
University of Illinois at Chicago
,
2
Department of Animal Science
,
University of Illinois at Urbana-Champaign
Chapters
00:04
Title
01:16
Setting Up and Incubating the ITO (Indium Tinoxide)-treated Slide for Cocultures
04:04
Drying Slide and Preparing for MALDI-TOF MS (Matrix-assisted Laser Desorption/Ionization Mass Spectrometry)
05:48
Results: Proper Sample Preparation and Representative IMS Data
07:16
Conclusion
Summary
Automatic Translation
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Automatic Translation
提出了一种新的样品制备方法, 以适应细胞和组织共培养, 以检测小分子交换使用成像质谱。
Tags
Small Molecules
Imaging Mass Spectrometry
Ovarian Cancer
Metastasis
Spatial Analysis
Cell-cell Communication
Agarose
Cell Culture
Co-culture
Label-free
Therapeutic Targets
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